Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM 2 articles published in JoVE Medicine Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development Gloria Pegoli1,2, Federica Lucini1,2, Chiara Mozzetta3, Chiara Lanzuolo1,2,4 1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", 2IRCCS Santa Lucia Foundation, 3Department of Biology and Biotechnology, "C. Darwin" Sapienza University, CNR - IBPM, 4CNR – Institute of Biomedical Technologies (ITB) Here, we propose a method to efficiently obtain single muscle fibers at early post-natal developmental stages from homozygous mutant Lamin Δ8-11 mouse model, a very severe model for Emery-Dreifuss muscular dystrophy (EDMD). Genetics 3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells Federica Marasca1, Alice Cortesi1, Lara Manganaro1, Beatrice Bodega1 1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM 3D multicolor DNA FISH represents a tool to visualize multiple genomic loci within 3D preserved nuclei, unambiguously defining their reciprocal interactions and localization within the nuclear space at a single cell level. Here, a step by step protocol is described for a wide spectrum of human primary cells.
